TY - JOUR AB - Our previous studies demonstrated a high fat diet-resistant lean phenotype of vitamin D receptor (VDR)-null mutant mice mainly due to increased energy expenditure, suggesting an involvement of the VDR in energy metabolism. Here, we took a transgenic approach to further define the role of VDR in adipocyte biology. We used the aP2 gene promoter to target the expression of the human (h) VDR in adipocytes in mice. In contrast to the VDR-null mice, the aP2-hVDR Tg mice developed obesity compared with the wild-type counterparts without changes in food intake. The increase in fat mass was mainly due to markedly reduced energy expenditure, which was correlated with decreased locomotive activity and reduced fatty acid beta-oxidation and lipolysis in the adipose tissue in the transgenic mice. Consistently, the expression of genes involved in the regulation of fatty acid transport, thermogenesis, and lipolysis were suppressed in the transgenic mice. Taken together, these data confirm an important role of the VDR in the regulation of energy metabolism. AD - Committee on Molecular Metabolism and Nutrition, Division of Biological Sciences,The University of Chicago, Chicago, Illinois 60637, USA. AN - 21840998 AU - Wong, K. E. AU - Kong, J. AU - Zhang, W. AU - Szeto, F. L. AU - Ye, H. AU - Deb, D. K. AU - Brady, M. J. AU - Li, Y. C. C2 - 3190828 DA - Sep 30 DO - 10.1074/jbc.M111.257568 ET - 2011/08/16 J2 - The Journal of biological chemistry KW - Adipocytes/*metabolism/physiology Animals Biological Transport, Active/genetics *Energy Metabolism Fatty Acid-Binding Proteins/genetics/metabolism Fatty Acids/*metabolism *Gene Expression Regulation Humans Lipolysis/genetics Locomotion/genetics Mice Mice, Mutant Strains Mice, Transgenic Obesity/genetics/*metabolism/pathology Organ Specificity Oxidation-Reduction Promoter Regions, Genetic/genetics Receptors, Calcitriol/*biosynthesis/genetics Thermogenesis/genetics LA - eng M1 - 39 M3 - Research Support, N.I.H., Extramural N1 - Wong, Kari E Kong, Juan Zhang, Wenshuo Szeto, Frances L Ye, Honggang Deb, Dilip K Brady, Matthew J Li, Yan Chun 5P30CA014599-36/CA/NCI NIH HHS/ 5P60DK020595-34/DK/NIDDK NIH HHS/ R01HL085793/HL/NHLBI NIH HHS/ T32DK07074/DK/NIDDK NIH HHS/ J Biol Chem. 2011 Sep 30;286(39):33804-10. Epub 2011 Aug 12. PY - 2011 SN - 1083-351X (Electronic) 0021-9258 (Linking) SP - 33804-10 ST - Targeted expression of human vitamin D receptor in adipocytes decreases energy expenditure and induces obesity in mice T2 - J Biol Chem TI - Targeted expression of human vitamin D receptor in adipocytes decreases energy expenditure and induces obesity in mice UR - http://www.ncbi.nlm.nih.gov/pubmed/21840998 VL - 286 ID - 133 ER -